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Figure X Cannabinoid synthase pathway genes of Cannabis sativa strain ERBxHO40. Panel A

Chromosome 7 included a single high stringency match for either tetrahydrocannabinolic acid synthase or cannabidiolic acid synthase, associated with chemotype, as well as a population of low stringency matches. For each diploid plant’s chromosome (x-axis) colored points indicate the location of BUSCO (Benchmarking Universal Single-Copy Orthologs) genes for chromosome 7 (elevation for each point is in million base pairs). Lines connect identical BUSCO genes among neighboring samples. Changes in the elevation and spacing among BUSCO genes reflects structural variation within samples, while the lines draw attention to variability among each sample’s immediate neighbor. Triangles mark the location of matches for three cannabinoid synthases (CBCAS, CBDAS, and THCAS). Red triangles indicate high stringency matches to THCAS (BLASTN query of NCBI accession “AB212829.1”, percent identity >= 0.98, number of gaps = 0). Blue triangles indicate high stringency matches to CBDAS (BLASTN query of NCBI accession “AB292682.1”, percent identity >= 0.98, number of gaps = 0). Yellow triangles indicate high stringency matches to CBCAS (BLASTN query of NCBI accession “LY658671.1”, percent identity >= 0.98, number of gaps = 0). Gray triangles indicate low stringency matches to cannabinoid synthases (BLASTN query of cannabichromenic acid synthase (LY658671.1), cannabidiolic acid synthase (AB292682, AB292683, AB292684), and tetrahydrocannabinolic acid synthase (AB212829, AB212830), percent identity >= 0.90 and < 0.98, number of gaps > 0). Each plant that was characterized as predominantly producing THCA (Chemotype I) consisted of a pair of chromosomes (a and b) that each had a single match to tetrahydrocannabinolic acid synthase (AB212829.1), indicated by a single red triangle. Each plant that was characterized as predominantly producing CBDA (Chemotype III) consisted of a pair of chromosomes (a and b) that each had a single match to cannabidiolic acid synthase (AB292682.1), indicated by a single blue triangle. Each plant that was characterized as producing both THCA and CBDA (Chemotype II) consisted of a pair of chromosomes where one chromosome included a single match to tetrahydrocannabinolic acid synthase (AB212829.1), indicated by a single red triangle, and the other chromosome included a single match to cannabidiolic acid synthase (AB292682.1), indicated by a single blue triangle. Plants that were characterized as being cannabigerolic acid accumulators (Chemotype IV) included chromosomes that may have had a single match to tetrahydrocannabinolic acid synthase (AB212829.1) or no high stringency matches to any of the three assayed cannabinoid synthases. Gray triangles indicate that a substantial population of low stringency matches were observed on all of the chromosome sevens.